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ESTROGENS, E1 + E2 FRACTIONATED, SERUM

test app

 

 
TEST NAME: ESTROGENS, E1+E2, FRACTIONATED
Includes Estrone (E1) & Estradiol (E2)
LAB DEPT: Core Lab Sendouts
 
SPECIMEN REQUIREMENTS
Specimen Type:
Blood
Container/Tube
 
Preferred/Acceptable:
Plain, red-top Vacutainer®.
 
Collection Volume:
2.5 mL (minimum 1.4 mL)
 
Patient Preparation:
None
 
Sample Rejection:
Specimens collected in serum gel (SST) tubes
Mislabeled or unlabeled specimens.
 
Special Instructions:
None
 
LOGISTICS
Test Availability:
Daily
Turnaround Time:
3-6 days
Test set-up:
Monday-Saturday
Lab Testing Section:
Send-outs
Referred to:
Mayo Medical Laboratories (MMLI) / Test ID: ESTF
 
INTERPRETIVE
Reference Range:
ESTRONE (E1)
CHILDREN*
1-14 days: Estrone levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.
Males
Tanner Stages#
Mean Age
Reference Range
Stage I (>14 days and prepubertal)
7.1 years
Undetectable-16 pg/mL
Stage II
11.5 years
Undetectable-22 pg/mL
Stage III
13.6 years
10-25 pg/mL
Stage IV
15.1 years
10-46 pg/mL
Stage V
18 years
10-60 pg/mL
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (+/- 2) years. For boys there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
Females
Tanner Stages#
Mean Age
Reference Range
Stage I (>14 days and prepubertal)
7.1 years
Undetectable-29 pg/mL
Stage II
10.5 years
10-33 pg/mL
Stage III
11.6 years
15-43 pg/mL
Stage IV
12.3 years
16-77 pg/mL
Stage V
14.5 years
17-200 pg/mL
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (+/- 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
*The reference ranges for children are based on the published literature(1,2), cross-correlation of our assay with assays used to generate the literature data and on our data for young adults.
ADULTS
Males: 10-60 pg/mL
Females
Premenopausal: 17-200 pg/mL
Postmenopausal: 7-40 pg/mL
Conversion factor
E1: pg/mL x 3.704=pmol/L (molecular weight=270)
 
ESTRADIOL (E2)
CHILDREN*
1-14 days: Estradiol levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.
Males
Tanner Stages#
Mean Age
Reference Range
Stage I (>14 days and prepubertal)
7.1 years
Undetectable-13 pg/mL
Stage II
12.1 years
Undetectable-16 pg/mL
Stage III
13.6 years
Undetectable-26 pg/mL
Stage IV
15.1 years
Undetectable-38 pg/mL
Stage V
18 years
10-40 pg/mL
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (+/- 2) years. For boys there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
Females
Tanner Stages#
Mean Age
Reference Range
Stage I (>14 days and prepubertal)
7.1 years
Undetectable-20 pg/mL
Stage II
10.5 years
Undetectable-24 pg/mL
Stage III
11.6 years
Undetectable-60 pg/mL
Stage IV
12.3 years
15-85 pg/mL
Stage V
14.5 years
15-350 pg/mL**
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (+/- 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
*The reference ranges for children are based on the published literature(1,2), cross-correlation of our assay with assays used to generate the literature data and on our data for young adults.
ADULTS
Males: 10-40 pg/mL
Females
Premenopausal: 15-350 pg/mL**
Postmenopausal: <10 pg/mL
**E2 levels vary widely through the menstrual cycle.
Conversion factor
E2: pg/mL x 3.676=pmol/L (molecular weight=272)
 
Critical Values:
None established
Limitations:
NA
Methodology:
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Test Classification:
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
 
ADDITIONAL INFORMATION
For SLCH Laboratory use only
Lab Processing instructions:
Centrifuge specimen to separate within 2 hours of collection. Aliquot sample into a 5 mL screw-capped, plastic vial for transport.
 
Processed Volume:
1.2 mL (minimum 0.7 mL) serum
 
Transport Temperature:
Refrigerated
 
Analyte Stability:
Ambient (unprocessed): NA
PROCESSED:
Ambient: 28 days
Refrigerated: 28 days
Frozen: 28 days
 
Notes:
Reject if collected in a serum gel (Gold/Red SST) vacutainer
 
GENERAL INFORMATION
EPIC order name:
Estrogens, Fractionation  (EPIC Test# LAB980)
Cerner order name:
Estrogen E1 + E2 frc
CPT Codes:
82671 - Estrogens, Fractionated
82670 - Estradiol (if applicable)
82679 - Estrone (if applicable)
LOINC Value:
53766-2
Synonyms:
 
Last Updated: 11/24
Last Reviewed: 11/24