NEUROTRANSMITTER PROFILE 3, SPINAL FLUID

test app

TEST NAME: NEUROTRANSMITTER PROFILE 3, CSF

Includes: 5HIAA, HVA, 3OMD, Tetrahydrobiopterin/Neopterin, and 5-Methyltetrahydrofolate

LAB DEPT: Core Lab Sendouts

SPECIMEN REQUIREMENTS

Specimen Type:	CSF
Container/Tube
Preferred/Acceptable:	Medical Neurogenetics collection kit required. Each collection kit contains 5 microcentrifuge tubes. Call SLCH Lab at 314-454-4268 for special kit and sample collection/processing information.
Collection Volume:	Total of 4.5 mL CSF CSF should be collected from the first drop into the tubes in the numbered order. Fill each tube to the marked line with the required volumes. Tube 1: 0.5 mL Tube 2: 1.0 mL Tube 3: 1.0 mL (contains antioxidants necessary to protect the sample integrity) Tube 4: 1.0 mL Tube 5: 1.0 ml -If samples are not blood contaminated, the tubes should be placed on dry ice at bedside -If samples are blood contaminated, the tubes should immediately be sent to lab for processing prior to freezing.
Patient Preparation:	None
Sample Rejection:	CSF NOT collected in special collection kit. Mislabeled or unlabeled specimens.
Special Instructions:	1) - If samples not blood contaminated, sample should be placed on dry ice at bedside. If samples are blood contaminated, the tubes should immediately be sent to lab for processing. 2) Send a completed "Medical Neurogenetics, LLC" request form with specimens. The following three tests should be marked on requisition: Neurotransmitter Metabolites, Tetrahydrobiopterin, and 5-Methyltetrahydrofolate. Also include sample date, date of birth, current medications and relevant history. 3) Label tubes with patient name and ID number, leaving the tube number viewable. 4) Place samples inside a specimen transport bag and the Medical Neurogenetics, LLC request form inside the pouch of the transport bag.

LOGISTICS

Test Availability:	Daily
Turnaround Time:	15-19 days
Test set-up:	Monday-Friday
Lab Testing Section:	Send-outs
Referred to:	Mayo Medical Laboratories to Medical Neurogenetics / Test ID: FNTSM

INTERPRETIVE

Reference Range:	5-Methyltetrahydrofolate Age 5MTHF (years) (nmol/L) 0-0.2 40-240 0.2-0.5 40-240 0.5-2.0 40-187 2.0-5.0 40-150 5.0-10 40-128 10-15 40-120 Adults 40-120 Neurotransmitter Metabolites/Amines Age 5HIAA HVA 3-O-MD (years) (nmol/L) (nmol/L) (nmol/L) 0-0.2 208-1159 337-1299 <300 0.2-0.5 179-711 450-1132 <300 0.5-2.0 129-520 294-1115 <300 2.0-5.0 74-345 233-928 <150 5.0-10 66-338 218-852 <100 10-15 67-189 167-563 <100 Adults 67-140 145-324 <100 Tetrahydrobiopterin/Neopterin Profile Age BH4 Neop (years) (nmol/L) (nmol/L) 0-0.2 40-105 7-65 0.2-0.5 23-98 7-65 0.5-2.0 18-58 7-65 2.0-5.0 18-50 7-65 5.0-10 9-40 7-40 10-15 9-32 8-33 Adults 10-30 8-28 Note: If test results are inconsistent with the clinical presentation, please call reference laboratory to discuss the case and/or submit a second sample for confirmatory testing.
Critical Values:	None established
Limitations:	NA
Methodology:	High Performance Liquid Chromatography-Electrochemistry & Fluorescences
Test Classification:	HPLC testing was developed and its performance characteristics determined by Medical Neurogenetic, LLC. These HPLC test have not been cleared or approved by the U.S. Food and Drug Administration.

ADDITIONAL INFORMATION

For SLCH Laboratory use only

Lab Processing instructions:	If samples are blood contaminated, the tubes should immediately be centrifuged and the clear CSF transferred to new similarly labeled tubes, then immediately placed in dry ice to freeze. If samples are not blood contaminated, they should have been placed directly into dry ice at bedside. If not, place in dry ice immediately to freeze.
Processed Volume:	5 microcentrifuge tubes with each tube filled to marked line. (container 1 requires 0.5 mL while tubes 2, 3, 4 & 5 require 1.0 mL CSF)
Transport Temperature:	Frozen
Analyte Stability:	Ambient: Not stable Refrigerated: 24 hours Frozen: 72 hours @ -20ºC Indefinitely @ -80ºC
Notes:	1.	Send a completed "Neurochemistry Test Request Form" with specimen.
	2.	Collection kits and instructions are stored in refrigerator 1 in core lab.
	3.	Specimen should remain on dry ice until Mayo pick-up (do NOT freeze/thaw)
	4.	Specimen may also be used for succinyladenosine if requested.

GENERAL INFORMATION

EPIC order name: Neuropathy Profile 111 (EPIC Test# LAB8420)
Cerner order name:	NeuroProf 3
CPT Codes:	82542 = 5-Methyltetrahydofolate 82542 = Tetrahydrobiopterin/Neopterin 82542, 83497, 83150 = Neurotransmitter Metabolites/Amines
LOINC Value:	5-Hydroxyindoleacetic acid: 47544-2 Homovanillic Acid: 40846-8 3-O-methyldopa: 3496-7 5-Methyltetrahydrofolate: 47547-5 Tetrahydrobiopterin: 59247-7 Neopterin: 34146-1 Interpretation: In process
Synonyms:
Last Updated: 09/24
Last Reviewed: 09/24