5-HYDROXYINDOLEACETIC ACID (5HIAA), URINE

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TEST NAME: 5-HYDROXYINDOLEACETIC ACID (5-HIAA), URINE

LAB DEPT: Core Lab Sendouts

SPECIMEN REQUIREMENTS

Specimen Type:	Urine, 24Hr collection
Container/Tube
Preferred/Acceptable:	Brown, 24 hour urine collection jug with preservative added prior to start of collection. Call lab at 314-454-4268 to obtain collection jug with preservative.
Collection Volume:	Entire specimen collected over a 24-hour period.
Patient Preparation:	For 48 hours prior to, as well as during the collection, the patient should: limit the following to 1 serving per day: - Fruits - Vegetables - Nuts - Caffeinated beverages or foods. If clinically feasible, discontinue the following medications at least 48 hours before specimen collection: - Acetaminophen (Tylenol or generic versions) - Aspirin - Antihistamines - Cough syrups - Cold and flu medications Patient should also avoid caffeinated beverages such as tea and coffee or caffeinated foods for 48 hours before and during specimen collection.
Sample Rejection:	Specimens other than 24 hour collection. Mislabeled or unlabeled specimens.
Special Instructions:	1.	Please note collection start and stop time on collection container.
	2.	Refrigerate specimen during collection and send to lab promptly at end of collection period.
	3.	Call 314-454-42686 to obtain collection jug with preservative.
	4.	Some prescription medications could interfere with test results. See limitations below for list of medications that can interfere with results to determine if they can be discontinued for at least 48 hours prior to and during collection.

LOGISTICS

Test Availability:	Daily
Turnaround Time:	3-5 days
Test set-up:	Monday-Friday
Lab Testing Section:	Send-outs
Referred to:	Mayo Medical Laboratories (MMLI) / Test ID: HIAA

INTERPRETIVE

Reference Range:

Reference Values

	5HIAA, mg/24 hour 99th percentile cutoff
Age	Female	Male
< or =23 months	< or =2.7	< or =2.3
24-35 months	< or =3.0	< or =2.6
3 years	< or =3.2	< or =2.9
4 years	< or =3.4	< or =3.2
5 years	< or =3.6	< or =3.6
6 years	< or =3.8	< or =3.9
7 years	< or =4.0	< or =4.2
8 years	< or =4.2	< or =4.5
9 years	< or =4.5	< or =4.8
10 years	< or =4.7	< or =5.1
11 years	< or =4.9	< or =5.3
12 years	< or =5.2	< or =5.6
13 years	< or =5.4	< or =5.8
14 years	< or =5.6	< or =6.1
15 years	< or =5.7	< or =6.3
16 years	< or =5.9	< or =6.4
17 years	< or =6.0	< or =6.6
18 years	< or =6.0	< or =6.7

call lab at 314-454-4268 to get reference values for ages >18 years

Critical Values:

Not established

Limitations:

Intake of food with a high content of serotonin (avocados, dates, eggplant, all fruit [including bananas, cantaloupe, grapefruit, kiwifruit, melons, pineapple, plantains, plums], all nuts [including hickory nuts, butternuts, pecans, walnuts], and tomatoes and tomato products) within 48 hours of the urine collection could result in falsely elevated 5-hydroxyindoleacetic acid (5-HIAA) excretion.

Numerous drugs affect the excretion of 5-HIAA by different mechanisms, including increased serotonin synthesis, metabolism, and release and inhibition of uptake. The following medications can interfere with 5-HIAA results. If clinically feasible, discontinue the following medications at least 48 hours before specimen collection. Acetaminophen (Tylenol or generic versions), Aspirin, Antihistamines, Cough syrups, Cold and flu medications

Methodology:

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Test Classification:

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

ADDITIONAL INFORMATION

For SLCH Laboratory use only

Lab Processing instructions:	*Pre-collection: Add 25 mL of 50% acetic acid, as preservative, to collection container to be used. Label jug as containing preservative (acid). Use 15 mL of 50% acetic acid for children <5 years old. This preservative is intended to achieve a pH of between approximately 2.0 and 4.0. Post-collection*: When specimen received in lab, mix sample well and measure total volume collected. Aliquot urine into a plastic, 13-mL urine tube. Note total volume collected on aliquot container and in cerner.
Processed Volume:	5.0 mL (minimum 1.0 mL) urine.
Transport Temperature:	Refrigerated
Analyte Stability:	Ambient: NA Refrigerated: 56 days Frozen: 365 days
Notes:	NO random or spot collections are acceptable. 24 hour volume is required.

GENERAL INFORMATION

EPIC order name:	5 HIAA, Urine, Quantitative 24 Hour (EPIC Test# LAB352)
Cerner order name:	5HIAA 24H Ur
CPT Codes:	83497
LOINC Value:	1695-6
Synonyms:	5-OH-Indoleacetic Acid
Last Updated: 12/20
Last Reviewed: 06/22