ASPERGILLUS (GALACTOMANNAN) ANTIGEN, SERUM
test app
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Specimen Type:
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Blood |
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Container/Tube
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Preferred/acceptable:
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Gold SST Vacutainer® (serum gel tube).
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Collection Volume:
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3.0 mL (minimum 2.0 mL)
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Patient Preparation:
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None
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Sample Rejection:
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Specimens collected in Plain, Red-top Tubes.
Specimens that are grossly hemolyzed and/or grossly lipemic.
Mislabeled or unlabeled specimens.
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Special Instructions:
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DO NOT open tube as opening the tube could allow aspergillus in the air to contaminate the specimen. Avoid exposure of specimen to atmosphere. Specimen will be cancelled if collected in plain, red-top tube.
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Test Availability:
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Daily
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Turnaround Time:
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2-5 days
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Test set-up:
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Sunday-Friday
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Lab Testing Section:
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Send-outs
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Referred to:
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Mayo Medical Laboratories (MMLI) / Test ID: ASPAG
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Reference Range:
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<0.5 index
Reference values apply to all ages
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Critical Values:
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Not established
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Limitations:
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False-positive results are reported to occur at rates of 8% to 14% with this assay. Two or more consecutive positive results should be obtained from separately drawn specimens before the patient is considered to have a positive Aspergillus antigen test.
Semisynthetic antibiotics such as piperacillin, amoxicillin, and augmentin, which are based on natural compounds derived from the genus Penicillium, have been demonstrated to cross-react with the rat EBA-2 monoclonal antibody used in the assay.
The specificity of the assay for Aspergillus species cannot exclude the involvement of other fungal pathogens with similar clinical presentations such as Fusarium, Alternaria, and Mucorales.
The performance of the assay has not been evaluated with neonate serum specimens.
False-positive galactomannan results are possible in patients receiving PLASMA-LYTE for intravenous hydration or if PLASMA-LYTE is used for bronchoalveolar lavage.
Specimens containing Histoplama antigen may cross-react in the Apergillus galactomannan assay.
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Methodology:
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Enzyme Immunoassay (EIA)
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Test Classification:
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This test has been cleared, approved, or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
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Lab Processing instructions:
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Centrifuge specimen to separate.
DO NOT ALIQUOT from original container and DO NOT OPEN TOP from Vacutainer®.
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Processed Volume:
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1.5 mL (minimum 1.0 mL) serum.
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Transport Temperature:
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Refrigerated
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Analyte Stability:
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PROCESSED:
Ambient: NA
Refrigerated: 14 days
Frozen: 14 days
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Notes:
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Reject if collected in plain, red-top tube.
Reject due to gross hemolysis and/or gross lipemia
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EPIC order name:
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Aspergillus Galactomannan Antigen (EPIC Test# LAB1311)
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Cerner order name:
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Asper galacto Ag
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CPT Codes:
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87305
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LOINC Value:
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44357-2
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Synonyms:
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Last Updated: 09/19
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Last Reviewed: 11/23
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