SOLUBLE IL-2R

test app

TEST NAME: SOLUBLE IL-2R (SOLUBLE CD25)

LAB DEPT: Core Lab Sendouts

SPECIMEN REQUIREMENTS

COLLECT SPECIMEN FOR RECEIPT IN LAB DAY OF COLLECTION BY 16:30 P.M. DO NOT COLLECT BEFORE 10:00 A.M if possible.
Specimen Type:	Blood
Container/Tube
Preferred/Acceptable:	Lavender top (EDTA) Vacutainer®.
Collection Volume:	3.0 mL (minimum 1.0 mL)
Patient Preparation:	None
Sample Rejection:	Specimens collected in gel tubes. Mislabeled or unlabeled specimens.
Special Instructions:	1.	Immediately invert tube gently 8-10 times after collection to ensure proper mixing of blood and anticoagulant (this will avoid specimen clotting). Do not overfill tube
	2.	Send to lab promptly, keeping specimen at room temperature.
	3.	Complete a "Cincinnati Children's DIL test requisition form" and send with specimen.

LOGISTICS

Test Availability:	Monday-Sunday
Turnaround Time:	3-5 days
Test set-up:	Monday-Friday
Lab Testing Section:	Send-outs
Referred to:	Cincinnati Children's Diagnostic Immunology Laboratory

INTERPRETIVE

Reference Range:

<3 years:	398-1940 U/mL
3-7 years:	144-1329 U/mL
>=8 years:	137-838 U/mL

Critical Values:

None established

Limitations:

Basiliximab binds with the IL2R in vivo.

Results for patients on basiliximab reflect the unbound portion of sIL2R in a plasma sample.

Methodology:

Chemiluminescence

Classification:

This test was developed and its performance characteristics determined by the Cancer and Blood Diseases Institute Clinical Laboratories at CCHMC. It has not been cleared or approved by the US Food and Drug Administration.

ADDITIONAL INFORMATION

For SLCH Laboratory use only

Lab Processing instructions:	Send whole blood (DO NOT SPIN) Monday-Thursday ONLY. Keeping specimen at ambient temperature. If specimen is collected Friday-Sunday OR before 10 am any day, see notes below for processing instructions since whole blood will be unacceptable to be sent
Processed Volume:	3.0 mL (minimum 1.0 mL) whole blood
Transport Temperature:	Ambient
Analyte Stability:	Ambient: 24 hours Refrigerated: Not acceptable Frozen: 6 mo if processed (frozen whole blood not acceptable).
Notes:	Send a completed "CCH DIL - Test requisition" form with specimen. Specimens need to be received at CCH within 24 hours of collection (make sure specimen was not collected before 10:00 am as specimens will not be received at CCH before 1000 am the next day). Ship specimens Monday-Thursday ONLY, marking on outside of shipping box "Store at Room Temperature - DO NOT Refrigerate" and "Deliver Promptly Upon Arrival". if specimen collected friday-sunday or prior to 1000 am any day, spin and aliquot plasma into two 5-mL screw-cap plastic vials for transport (or one vial if only 0.5 mL plasma available). Place aliquot vial in dry ice to freeze immediately. Specimen must be kept frozen until able to ship and than shipped frozen on dry ice. Call or fax CBDI lab @ (513-636-4685/513-636-3861) to inform them specimen being shipped and provide tracking number. Ship samples to: CCHMC-Julie Beach; DIL-Room R2328, 3333 Burnet Ave., Cincinnati, OH 45229-3039

GENERAL INFORMATION

EPIC order name:	Soluble IL-2R (EPIC Test# LAB5749)
Cerner order name:	Soluble IL-2R
CPT Codes:	83520
LOINC Value:	43171-8
Synonyms:
Last Updated: 06/22
Last Reviewed: 01/25